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宿主来源
Mouse抗原名称
Alexa Fluor® 488克隆号
S-3378抗体类型
Mouse mAb抗体同种型
IgG1,k纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:1000 Chemical
Anti-Alexa Fluor® 488 antibody are designed to dependably detect Alexa Fluor® 488 in various applications.
免疫细胞化学
ICC analysis of Mouse Anti-Alexa Fluor® 488 Monoclonal Antibody was performed using HeLa cells. In the positive control: Anti-Histone H3 antibody (S0B0079) was used at 1/500 dilution and incubated overnight at 4C. Goat polyclonal Antibody to Rabbit lgG -H&L (Alexa Fluore 488) was used as secondary antibody at 1/1000 dilution for 1 hour at room temperature. Further, Alexa Fluor 488 signal was quenched by incubating the cells with Mouse Anti-Alexa Fluore 488 Monoclonal Antibody for 1 hour at room temperature. The cells were fixed with 100%ice-cold methanol and permeabilized with 0.1% PBS-Triton X 100. Nuclei were counterstained with DAPI(Blue).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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