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宿主来源
Rabbit抗原名称
Phospho-PKA C (Thr197)分子别名
cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PKACA; PRKACA免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P17612克隆号
S-1137-310抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt, Mk预测反应种属
(反应种属缩写表)Bv, C.el, Dg, Dr, Hm, Pg, Sh纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu
Phospho-PKA C (Thr197) is the catalytic subunit of cAMP-dependent protein kinase A that carries a single phosphate group on threonine 197 within its activation loop; this modification, catalyzed by the upstream kinase PDK1, locks the kinase in an active conformation, enhances substrate turnover, and serves as a biomarker of PKA pathway activation in response to hormones such as epinephrine and glucagon, making it a critical node in signal transduction, synaptic plasticity, and metabolic regulation.
免疫印迹
WB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDaWB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: phosphatase treated NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDaWB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: phosphatase treated C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDaWB result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
斑点杂交
Dot blot result of Phospho-PKA C (Thr197) Recombinant Rabbit mAb
Lane 1: PKA C (Thr197) phospho peptide
Lane 2: PKA C (Thr197) unmodified peptide
Primary antibody: Phospho-PKA C (Thr197) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of HeLa cells starved overnight, then treated with Calyculin A (100nM, 30 min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-PKA C (Thr197) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
引用文献(1)
- Structural characterization of red yeast rice-derived polysaccharide and its promotion of lipid metabolism and gut function in high-fat diet-induced mice
Luming Qi,Zhixuan Chen,Di Wang, Lisha Wang,Mohamed Mohamed Soliman, Salah M El-Bahy, Zhanhu Guo, Zeinhom M El-Bahy, Maomao Zhang, Peng Hu, Kui Zhao
Int J Biol Macromol. 2024 Oct 19 .
影响因子： 8.5
货号：S0B6265产品名称：Phospho-PKA C (Thr197) Recombinant Rabbit mAb (S-1137-310)
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