RBPMS Recombinant Rabbit mAb (S-2387-163)

RNA-binding protein with multiple splicing,RBP-MS,Heart and RRM expressed sequence (Hermes),HERMES

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6263

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

RBPMS
分子别名

RNA-binding protein with multiple splicing; RBP-MS; Heart and RRM expressed sequence (Hermes); HERMES
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q93062
克隆号

S-2387-163
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HepG2, A549, A431, HeLa, mouse heart, rat heart
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
IF 1:500 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
IF	1:500	Ms

背景介绍

RBPMS (RNA-binding protein with multiple splicing) is a vertebrate-conserved RNA-binding protein that harbors a single, ~80-aa RNA-recognition motif (RRM) flanked by N- and C-terminal extensions rich in prolines, is expressed as numerous mRNA and protein isoforms from a >230-kb locus, and functions as a master post-transcriptional regulator by directing alternative splicing, stability and translation of target mRNAs critical for cardiac, smooth-muscle, retinal ganglion cell and oocyte development, as well as for DNA-damage responses through interactions with RIF1 and modulation of TGF-β/BMP signaling pathways.

免疫印迹
- WB result of RBPMS Recombinant Rabbit mAb
  Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: U-937 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Lane 5: HeLa whole cell lysate 20 µg
  Low expression control: U-937 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22-28 kDa
  This blot was developed with high sensitivity substrate
- WB result of RBPMS Recombinant Rabbit mAb
  Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22-28 kDa
  This blot was developed with high sensitivity substrate
- WB result of RBPMS Recombinant Rabbit mAb
  Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22-28 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in A549 cells. Anti- RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
- IF shows positive staining in paraffin-embedded mouse retina. Anti- RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.