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SEK1/MKK4 Rabbit Polyclonal Antibody

Dual specificity mitogen-activated protein kinase kinase 4,MAP kinase kinase 4,MAPKK 4,JNK-activating kinase 1,MAPK/ERK kinase 4 (MEK 4),SAPK/ERK kinase 1 (SEK1),Stress-activated protein kinase kinase 1 (SAPK kinase 1,SAPKK-1,SAPKK1),c-Jun N-terminal kinase kinase 1 (JNKK),JNKK1,MEK4,PRKMK4,SERK1,SKK1,MAP2K4

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B6262

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

SEK1/MKK4
分子别名

Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4 (MEK 4); SAPK/ERK kinase 1 (SEK1); Stress-activated protein kinase kinase 1 (SAPK kinase 1; SAPKK-1; SAPKK1); c-Jun N-terminal kinase kinase 1 (JNKK); JNKK1; MEK4; PRKMK4; SERK1; SKK1; MAP2K4
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P45985
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HEK-293, HeLa, K562, Jurkat, NIH/3T3, mouse brain, C6, rat brain, COS-7
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
IHC-P 1:200 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu

背景介绍

SEK1/MKK4 (also known as MAP2K4, MEK4, JNKK1) is a 399-amino-acid dual-specificity protein kinase located on chromosome 17p11.2 that functions as the immediate upstream activator of both JNK and p38 MAPKs in response to diverse extracellular stresses such as osmotic shock, UV radiation, and cytokines; after phosphorylation by upstream MAP3Ks like MEKK1/2, SEK1/MKK4 phosphorylates JNK and p38 on their Thr-Pro-Tyr motifs, thereby linking stress signals to transcriptional and apoptotic programs, and its activity and stability are subjected to negative feedback control through JNK-mediated phosphorylation of the E3 ubiquitin ligase Itch, which then ubiquitinates and targets SEK1/MKK4 for proteasomal degradation .

免疫印迹
- WB result of SEK1/MKK4 Rabbit pAb
  Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 40 kDa
- WB result of SEK1/MKK4 Rabbit pAb
  Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 42 kDa
- WB result of SEK1/MKK4 Rabbit pAb
  Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 42 kDa
- WB result of SEK1/MKK4 Rabbit pAb
  Primary antibody: SEK1/MKK4 Rabbit pAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 42 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SEK1/MKK4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of SEK1/MKK4 in tumor tissue.
- Expression of SEK1/MKK4 in human tissue.