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BMAL1 Recombinant Rabbit mAb (S-1918-155)

Basic helix-loop-helix ARNT-like protein 1,Aryl hydrocarbon receptor nuclear translocator-like protein 1,Basic-helix-loop-helix-PAS protein MOP3,Brain and muscle ARNT-like 1,Class E basic helix-loop-helix protein 5 (bHLHe5),Member of PAS protein 3,ARNTL,BHLHE5,MOP3,PASD3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6257

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

BMAL1
分子别名

Basic helix-loop-helix ARNT-like protein 1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5 (bHLHe5); Member of PAS protein 3; ARNTL; BHLHE5; MOP3; PASD3
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

O00327
克隆号

S-1918-155
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, SH-SY5Y, PC-3, Raji, NIH/3T3, mouse liver, rat liver
预测反应种属
(反应种属缩写表)

Or,Hr,Hm,Ck
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ChIP	1:20-1:50	Hu

背景介绍

BMAL1 (Brain and muscle ARNT-like 1, also known as ARNTL) is a bHLH/PAS-domain transcription factor that forms a heterodimer with CLOCK (or NPAS2) to drive the transcription of clock-controlled genes (e.g., Per and Cry) via E-box elements, thereby operating as the positive limb of the circadian transcription-translation feedback loop essential for generating ~24-hour molecular rhythms; it is the only clock gene whose loss abolishes circadian rhythmicity in mammals and whose disruption is linked to a spectrum of disorders including metabolic syndrome, psychiatric and neurodegenerative diseases, infertility, and altered sleep, while also regulating adipogenesis, immune responses, embryonic development, and protein synthesis through interactions with metabolic and signaling pathways.

免疫印迹
- WB result of BMAL1 Recombinant Rabbit mAb
  Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Lane 3: PC-3 whole cell lysate 20 µg
  Lane 4: Raji whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 70 kDa
- WB result of BMAL1 Recombinant Rabbit mAb
  Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 70 kDa
- WB result of BMAL1 Recombinant Rabbit mAb
  Primary antibody: BMAL1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 70 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- BMAL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti- BMAL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used BMAL1 Recombinant Rabbit mAb (S-1918-155) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of PER1 and SAT-α in BMAL1 Recombinant
  Rabbit mAb (S-1918-155)-immunoprecipitated sample.