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Rabbit抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; PTPRC细胞定位
Cell membrane, SynapseAccession
P08575克隆号
S-3361抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
J774A.1, RAW264.7, moues spleen, mouse thymus纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:1000 Ms ICC 1:500 Ms IF 1:500 Ms
CD45, also known as protein tyrosine phosphatase receptor type C (PTPRC), is a crucial transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells except erythrocytes and plasma cells. It is a receptor-like protein tyrosine phosphatase that plays a vital role in regulating immune cell activation and differentiation. CD45 consists of an extracellular domain, a transmembrane segment, and intracellular phosphatase domains. Its primary function is to dephosphorylate specific tyrosine residues on target proteins, thereby modulating signaling pathways essential for immune responses. CD45 has multiple isoforms generated by alternative splicing, which are differentially expressed across immune cell subsets and influence their activation thresholds. For example, naïve T cells predominantly express CD45RA, while memory T cells express CD45RO, which enhances T-cell receptor signaling. In pathology, CD45 is used as a marker to identify hematopoietic cells and distinguish lymphomas from other types of cancer.
免疫印迹
WB result of CD45 Recombinant Rabbit mAb
Primary antibody: CD45 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: J774A.1 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 148 kDa
Observed MW: 200 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD45 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-CD45 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in RAW 264.7 cells. Anti- CD45 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD45 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CD45 in tumor tissue.
Expression of CD45 in human tissue.
Expression of CD45 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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