分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Cleaved Caspase-8 (Asp387)分子别名
CASP-8; Casp8细胞定位
Cytoplasm, NucleusAccession
O89110克隆号
S-2953抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Ms ICC 1:500 Ms
Cleaved Caspase-8 (Asp387) is the ~18 kDa C-terminal catalytic fragment generated by autoproteolysis at Asp387 during death-receptor-mediated apoptosis, retaining the active-site cysteine and small subunit that, once released from the DISC and freed from its prodomain, forms a heterotetrameric protease capable of cleaving downstream caspases and substrates such as Bid, thereby amplifying the extrinsic apoptotic signaling cascade.
免疫印迹
WB result of Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated CTLL-2 whole cell lysate 20 µg
Lane 2: CTLL-2 treated with 10 μg/ml cycloheximide overnight, then treated with 20 ng/ml mTNF-α for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 18, 43 kDa
免疫细胞化学
ICC analysis of RAW264.7 cells treated with Cycloheximide(2.5ug/ml,4h) and mTNF-α (20ng/ml,4h) (top panel) and untreated RAW264.7 cells (below panel). Anti- Cleaved Caspase-8 (Asp387) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)