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NF-κB p105/p50 Recombinant Rabbit mAb (S-3354)

Nuclear factor NF-kappa-B p105 subunit,DNA-binding factor KBF1,EBP-1,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1,NFKB1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6247

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

NF-κB p105/p50
分子别名

Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; NFKB1
细胞定位

Cytoplasm, Nucleus
Accession

P19838
克隆号

S-3354
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, Jurkat, THP-1, Raji, MCF7, Daudi, A20, mouse spleen, rat spleen
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms, Rt
IHC-P 1:500 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

The NF-κB p105 precursor protein is a 105 kDa cytoplasmic ankyrin-repeat-rich molecule that undergoes stimulus-dependent, ubiquitin/proteasome-mediated processing to liberate the 50 kDa DNA-binding p50 subunit, which then dimerizes with other NF-κB/Rel members to form transcriptionally active complexes that rapidly enter the nucleus and regulate genes governing immunity, inflammation, cell survival, and proliferation; p105 itself also serves as an IκB-like inhibitor by sequestering associated dimers in the cytoplasm, and its balanced processing versus full-length stability is tightly controlled by IKK-mediated phosphorylation, ensuring dynamic NF-κB signaling in response to cytokines, pathogens, and cellular stresses.

免疫印迹
- WB result of NF-kB p105/p50 Recombinant Rabbit mAb
  Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: THP-1 whole cell lysate 20 µg
  Lane 4: Raji whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Lane 6: Daudi whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 105 kDa
  Observed MW: 50, 105 kDa
- WB result of NF-kB p105/p50 Recombinant Rabbit mAb
  Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: A20 whole cell lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 105 kDa
  Observed MW: 50, 105 kDa
- WB result of NF-kB p105/p50 Recombinant Rabbit mAb
  Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 105 kDa
  Observed MW: 50, 105 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+hTNF-α (30 ng/ml, 1 hr) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used NF-kB p105/p50 Recombinant Rabbit mAb (S-3354) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of IL-8 and SAT-α in NF-kB p105/p50
  Recombinant Rabbit mAb (S-3354)-immunoprecipitated sample.
组织表达图谱
- Expression of NF-κB p105/p50 in tumor tissue.