NOX4 Recombinant Rabbit mAb (S-2246-45)

NADPH oxidase 4,Kidney oxidase-1 (KOX-1),Kidney superoxide-producing NADPH oxidase,Renal NAD(P)H-oxidase,RENOX

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6246

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

NOX4
分子别名

NADPH oxidase 4; Kidney oxidase-1 (KOX-1); Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; RENOX
免疫原

Synthetic Peptide
细胞定位

Nucleus, Endoplasmic reticulum membrane, Cell membrane, Cytoplasm
Accession

Q9NPH5
克隆号

S-2246-45
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HEK-293, BxPC-3, HeLa, U-87, Neuro-2a, PC-12, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
ICC	1:500	Hu

背景介绍

NOX4, a constitutively active member of the NADPH oxidase family, is widely expressed in human tissues (including kidney, heart, liver, and brain) and localizes primarily to the outer mitochondrial membrane, where it catalyzes the reduction of molecular oxygen to hydrogen peroxide (H₂O₂) without requiring cytosolic regulatory subunits; it associates only with p22phox and can be further activated by Poldip2. Functionally, NOX4-generated reactive oxygen species (ROS) regulate diverse cellular processes such as proliferation, differentiation, apoptosis, senescence, and mitochondrial dysfunction, and it contributes to pathologies including cancer (e.g., breast and kidney cancer), fibrosis, atherosclerosis, and stroke. Recent studies also reveal NOX4's presence in the nucleus of kidney cancer cells, where its expression predicts resistance to targeted therapies and poor survival .

免疫印迹
- WB result of NOX4 Recombinant Rabbit mAb
  Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled HEK-293 whole cell lysate 20 µg
  Lane 2: unboiled BxPC-3 whole cell lysate 20 µg
  Lane 3: unboiled HeLa whole cell lysate 20 µg
  Lane 4: unboiled U-87 MG whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 67 kDa
  Observed MW: 62 kDa
- WB result of NOX4 Recombinant Rabbit mAb
  Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 67 kDa
  Observed MW: 62 kDa
- WB result of NOX4 Recombinant Rabbit mAb
  Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 67 kDa
  Observed MW: 62 kDa
- WB result of NOX4 Recombinant Rabbit mAb
  Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 67 kDa
  Observed MW: 62 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- NOX4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).