Optineurin Recombinant Rabbit mAb (S-2030-40)
E3-14.7K-interacting protein (FIP-2),Huntingtin yeast partner L,Huntingtin-interacting protein 7 (HIP-7),Huntingtin-interacting protein L,NEMO-related protein,FIP2,GLC1E,HIP7,HYPL,NRP,OPTN
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宿主来源
Rabbit抗原名称
Optineurin分子别名
E3-14.7K-interacting protein (FIP-2); Huntingtin yeast partner L; Huntingtin-interacting protein 7 (HIP-7); Huntingtin-interacting protein L; NEMO-related protein; FIP2; GLC1E; HIP7; HYPL; NRP; OPTN免疫原
Synthetic PeptideAccession
Q96CV9克隆号
S-2030-40抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, A172, HDLM-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
Optineurin (OPTN) is a 67 kDa multifunctional cytosolic adaptor protein, originally identified as FIP-2 (E3-14.7K-interacting protein) and later implicated in normal-tension glaucoma, amyotrophic lateral sclerosis, Paget’s disease of bone, and Crohn’s disease. It contains several domains (e.g., leucine zipper, zinc finger, ubiquitin-binding domain, LC3-interacting region) enabling interaction with proteins such as Rab8, myosin VI, and TBK1, thus participating in vesicular trafficking, Golgi maintenance, NF-κB-mediated antiviral signaling, and autophagy (including aggrephagy, mitophagy, and xenophagy). Notably, OPTN acts as an autophagy receptor, recruiting ubiquitinated mitochondria to autophagosomes during mitophagy via its ubiquitin-binding activity, and its mutations (e.g., E50K, M98K) disrupt autophagy and lead to neurodegeneration and retinal ganglion cell death.
免疫印迹
WB result of Optineurin Recombinant Rabbit mAb
Primary antibody: Optineurin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A172 whole cell lysate 20 µg
Lane 4: HDLM-2 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 72 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Optineurin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Optineurin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Optineurin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Optineurin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Optineruin in tumor tissue.
Expression of Optineruin in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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