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Rabbit抗原名称
Phospho-CrkL (Tyr207)分子别名
Crk-like protein; CRKL免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P46109抗体类型
Polyclonal antibody抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu阳性样本
K562纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:100 Hu
Phospho-CrkL (Tyr207), the tyrosine-207-phosphorylated form of the CRK-like adaptor protein CrkL, is a 34 kDa signaling scaffold that contains SH2 and SH3 domains enabling it to bind multiple partners and propagate signals through MAPK, PI3K and STAT cascades; phosphorylation at Tyr207 (the CrkL counterpart of Tyr221 in CrkII) is a key regulatory switch controlling cytoskeletal reorganization, immune-cell signaling, cell proliferation and migration, and its dysregulation contributes to Philadelphia-chromosome-positive leukemias via constitutive activation with BCR-ABL, while developmental perturbations are linked to congenital heart defects, making p-CrkL(Tyr207) a focal point in oncogenic and developmental research .
免疫印迹
WB result of Phospho-CrkL (Tyr207) Rabbit pAb
Primary antibody: Phospho-CrkL (Tyr207) Rabbit pAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: phosphatase treated K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 38 kDa
免疫细胞化学
ICC shows positive staining in K562 cells. Anti- Phospho-CrkL (Tyr207) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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