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Rabbit抗原名称
PTP1B分子别名
Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B (PTP-1B); PTP1B; PTPN1免疫原
Recombinant Protein细胞定位
Endoplasmic reticulum membraneAccession
P18031抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HCT 116, HeLa, HepG2, A549, MCF7, Jurkat, SW480, TF-1, NIH/3T3, RAW264.7, mouse brain, mouse heart, mouse spleen, PC-12, rat brain, rat heart, rat spleen纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Protein tyrosine phosphatase 1B (PTP1B), encoded by the PTPN1 gene, is a 50 kDa non-receptor intracellular tyrosine phosphatase anchored to the cytoplasmic face of the endoplasmic reticulum that acts as a key negative regulator of both the insulin and leptin signaling cascades by dephosphorylating the insulin receptor, IRS-1, JAK2, and leptin receptor, thereby dampening downstream PI3K/AKT and STAT3 signaling; PTP1B-null mice are lean, insulin-hypersensitive, and resistant to diet-induced obesity, while elevated PTP1B expression is associated with insulin resistance, type 2 diabetes, obesity, and several cancers (especially breast and ovarian cancer), and selective PTP1B inhibitors are actively explored as therapeutics for metabolic and oncologic disorders.
免疫印迹
WB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Lane 7: SW480 whole cell lysate 20 µg
Lane 8: TF-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDaWB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Lane 5: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDaWB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat heart lysate 20 µg
Lane 4: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PTP1B in tumor tissue.
Expression of PTP1B in human tissue.
Expression of PTP1B in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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