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SATB1 + SATB2 Recombinant Mouse mAb (S-3382)

DNA-binding protein SATB1,Special AT-rich sequence-binding protein 1,DNA-binding protein SATB2,Special AT-rich sequence-binding protein 2

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6242

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
抗原名称

SATB1+SATB2
分子别名

DNA-binding protein SATB1; Special AT-rich sequence-binding protein 1; DNA-binding protein SATB2; Special AT-rich sequence-binding protein 2
细胞定位

Nucleus
Accession

Q01826, Q9UPW6
克隆号

S-3382
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu, Ms, Rt
阳性样本

HT-1080, Saso-2, NIH/3T3, normal mouse E18 foetal brain
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

SATB1 and SATB2 are evolutionarily related, chromatin-remodeling transcription factors that share a common CUT-domain architecture for high-affinity binding to matrix-attachment regions, yet they orchestrate distinct developmental programs: SATB1 acts as a global organizer of T-cell chromatin during thymocyte maturation and promotes breast-cancer metastasis through epithelial-to-mesenchymal transition, whereas SATB2 is indispensable for craniofacial patterning, cortical neuron identity, and osteoblast differentiation by forming higher-order chromatin loops that juxtapose enhancers of lineage-specific genes; both proteins recruit histone-modifying enzymes and the NuRD complex, but their mutually exclusive expression patterns and differential interaction partners explain why SATB1 depletion enhances neural differentiation while SATB2 loss yields cleft palate and intellectual disability, making the SATB1/SATB2 balance a critical epigenetic switch in immunity, neurodevelopment, and tumorigenesis.

免疫印迹
- WB result of SATB1 + SATB2 Recombinant Mouse mAb
  Primary antibody: SATB1 + SATB2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HT-1080 whole cell lysate 20 µg
  Lane 2: Saso-2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 100 kDa
  This blot was developed with high sensitivity substrate
- WB result of SATB1 + SATB2 Recombinant Mouse mAb
  Primary antibody: SATB1 + SATB2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: normal mouse E18 foetal brain lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 100 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in HT-1080 cells. Anti- SATB1 + SATB2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).