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SEMA5A Recombinant Rabbit mAb (S-2560-24)

Semaphorin-5A,Semaphorin-F (Sema F),SEMAF

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6241
规格
数量
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SEMA5A

  • 分子别名

    Semaphorin-5A; Semaphorin-F (Sema F); SEMAF

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Membrane

  • Accession

    Q13591

  • 克隆号

    S-2560-24

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    A549, U-87 MG, HaCaT, A431, mouse brain, rat brain

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:500-1:1000 Hu, Ms, Rt
背景介绍

  • SEMA5A (Semaphorin-5A) is a transmembrane glycoprotein encoded by the SEMA5A gene that belongs to the class 5 semaphorin subfamily, featuring an extracellular sema domain, seven thrombospondin type-1 repeats (TSR1-7), and a short cytoplasmic tail. Originally identified as an axon guidance cue, SEMA5A is bifunctional, either promoting or inhibiting axon growth and cell migration depending on context, and it binds plexin-B3 to regulate neural circuit development in the retina, hippocampus, cortex, and spinal cord. Beyond the nervous system, SEMA5A exhibits context-dependent roles in tumorigenesis (e.g., promoting pancreatic cancer invasion but suppressing lung adenocarcinoma proliferation and migration) and in immune modulation (e.g., driving inflammation in rheumatoid arthritis and chronic urticaria). Importantly, dysregulation or mutation of SEMA5A is genetically linked to neurodevelopmental disorders such as autism spectrum disorder, intellectual disability, and Parkinson’s disease, with reduced expression observed in autistic brains.

  • 免疫印迹

    • WB result of SEMA5A Recombinant Rabbit mAb
      Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: U-87 MG whole cell lysate 20 µg
      Lane 3: HaCaT whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 130 kDa
      This blot was developed with high sensitivity substrate

    • WB result of SEMA5A Recombinant Rabbit mAb
      Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 125 kDa
      This blot was developed with high sensitivity substrate

    • WB result of SEMA5A Recombinant Rabbit mAb
      Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 125 kDa
      This blot was developed with high sensitivity substrate

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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