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YB1 Recombinant Rabbit mAb (S-1748-144)

Y-box-binding protein 1,YB-1,CCAAT-binding transcription factor I subunit A (CBF-A),DNA-binding protein B (DBPB),Enhancer factor I subunit A (EFI-A),Nuclease-sensitive element-binding protein 1,Y-box transcription factor,NSEP1,YBX1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6238
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    YB1

  • 分子别名

    Y-box-binding protein 1; YB-1; CCAAT-binding transcription factor I subunit A (CBF-A); DNA-binding protein B (DBPB); Enhancer factor I subunit A (EFI-A); Nuclease-sensitive element-binding protein 1; Y-box transcription factor; NSEP1; YBX1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Secreted, Cytoplasm

  • Accession

    P67809

  • 克隆号

    S-1748-144

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, SW480, MCF7, SK-BR-3, T-47D, Jurkat, A549, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu
    ICFCM 1:50 Hu
背景介绍

  • Y-box-binding protein 1 (YB-1), encoded by YBX1, is a multifunctional nucleic-acid-binding member of the cold-shock domain family that shuttles between cytoplasm and nucleus (the latter upon stress); it acts as a transcription factor, co-activator or repressor by binding the Y-box (5′-CTGATTGG-3′) and other elements, regulates mRNA splicing, stability and translation by associating with mRNPs and modulating cap-dependent/IRES-driven initiation, participates in DNA repair and stress responses, and its overexpression and nuclear accumulation are linked to cancer proliferation, metastasis, multidrug resistance (via MDR1, EGFR, CCNA/B1, etc.) and poor prognosis, making it a diagnostic marker and therapeutic target.

  • 免疫印迹

    • WB result of YB1 Recombinant Rabbit mAb
      Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: SW480 whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: SK-BR-3 whole cell lysate 20 µg
      Lane 5: T-47D whole cell lysate 20 µg
      Lane 6: Jurkat whole cell lysate 20 µg
      Lane 7: A549 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 47 kDa

    • WB result of YB1 Recombinant Rabbit mAb
      Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 45 kDa

    • WB result of YB1 Recombinant Rabbit mAb
      Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 45 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeled with anti-YB1 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stoamch. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-YB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of YB1 in tumor tissue.

    • Expression of YB1 in human tissue.

    • Expression of YB1 in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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