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Rabbit抗原名称
GFP分子别名
Green fluorescent protein免疫原
Synthetic PeptideAccession
P42212克隆号
S-296-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A浓度
1 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:50 Species Independent
The green fluorescent protein (GFP) is a ~27 kDa protein composed of 238 amino acids, originally isolated from the jellyfish Aequorea victoria. Its core structure features a unique β-barrel fold with a central fluorescent chromophore (formed by the autocatalytic cyclization and oxidation of Ser65-Tyr66-Gly67). When excited by blue light at 395 nm (primary peak) or 475 nm (secondary peak), GFP emits green fluorescence at 509 nm without requiring additional cofactors. This property has made GFP a revolutionary tool in molecular and cellular biology, widely used for gene expression reporting, protein localization tracking, and dynamic cell imaging.
免疫细胞化学
ICC shows positive staining in Histone H3-GFP transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- GFP (Alexa Fluor® 488 Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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