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Rabbit抗原名称
Atg5分子别名
Autophagy protein 5; APG5-like; Apoptosis-specific protein; APG5L; ASP; ATG5细胞定位
CytoplasmAccession
Q9H1Y0克隆号
S-3181抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
PANC-1, U-87 MG, HCT 116, MCF7, HeLa, Saos-2, MEF, C2C12, C6, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
Autophagy-related protein 5 (ATG5), encoded by the ATG5 gene on chromosome 6, is a core autophagy protein that forms a complex with ATG12 and ATG16L1 (the Atg12-Atg5-Atg16 complex), which is essential for lipidation of LC3 (microtubule-associated protein 1 light chain 3B) and subsequent autophagosome elongation and maturation. Beyond autophagy, ATG5 also has non-autophagic roles, including promoting apoptosis via mitochondrial translocation and cytochrome c release, regulating immune responses (e.g., antigen presentation and lymphocyte development), and affecting hematopoietic stem cell maintenance. ATG5 deficiency (e.g., in knockout mice) is neonatal lethal, causing autophagy defects, neurodegeneration, and impaired fertility, while its overexpression extends lifespan. Additionally, ATG5 mutations are linked to human diseases (e.g., ataxia), and it plays a role in viral infections (e.g., HIV-1) by modulating host antiviral responses.
免疫印迹
WB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: Saos-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDaWB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDaWB result of Atg5 Recombinant Rabbit mAb
Primary antibody: Atg5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 56 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Atg5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Atg5 in tumor tissue.
Expression of Atg5 in human tissue.
Expression of Atg5 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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