分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Atg13分子别名
Autophagy-related protein 13; KIAA0652; ATG13免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O75143克隆号
S-2534-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, PANC-1, Jurkat, MCF7, NIH/3T3, mouse brain, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Atg13 is an essential scaffold protein in the autophagy-initiation ULK1 complex, where it links the kinase ULK1 to FIP200 and Atg101 to form a stable platform that integrates nutrient-dependent signals from mTORC1 and AMPK; upon starvation, dephosphorylation of Atg13 releases mTORC1 inhibition, allowing ULK1 to phosphorylate Atg13 itself and other downstream targets to trigger phagophore nucleation, while constitutive phosphorylation of Atg13 under nutrient-rich conditions keeps autophagy suppressed, and mutations or depletion of Atg13 in model organisms cause severe defects in bulk and selective autophagy, underscoring its central role in maintaining cellular homeostasis through dynamic regulation of autophagic flux.
免疫印迹
WB result of Atg13 Recombinant Rabbit mAb
Primary antibody: Atg13 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 65 kDaWB result of Atg13 Recombinant Rabbit mAb
Primary antibody: Atg13 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 65 kDaWB result of Atg13 Recombinant Rabbit mAb
Primary antibody: Atg13 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Atg13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Atg13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Atg13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Atg13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Atg13 in tumor tissue.
Expression of Atg13 in human tissue.
Expression of Atg13 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)