所有图片（1/3）

HIF-1α Recombinant Rabbit mAb (S-3123)

Hypoxia-inducible factor 1-alpha,HIF-1-alpha,HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78 (bHLHe78),Member of PAS protein 1,PAS domain-containing protein 8,BHLHE78,MOP1,PASD8,HIF1A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6230

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

HIF-1α
分子别名

Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS protein 1; PAS domain-containing protein 8; BHLHE78; MOP1; PASD8; HIF1A
细胞定位

Cytoplasm, Nucleus
Accession

Q16665
克隆号

S-3123
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu,Ms,Rt
ICC 1:500 Hu,Ms,Rt
ChIP 1:20-1:50 Hu,Ms,Rt

应用	稀释度	推荐种属
WB	1:1000	Hu,Ms,Rt
ICC	1:500	Hu,Ms,Rt
ChIP	1:20-1:50	Hu,Ms,Rt

背景介绍

HIF-1α (hypoxia-inducible factor-1 alpha) is a crucial transcriptional regulator encoded by the HIF1A gene, playing a vital role in cellular adaptation to low-oxygen conditions. Under normal oxygen levels, HIF-1α has a short half-life and is rapidly degraded via the von Hippel-Lindau tumor suppressor gene product (pVHL)-mediated ubiquitin-proteasome pathway. However, in hypoxic conditions (when oxygen levels are below 5%), HIF-1α becomes stable, accumulates, and translocates to the nucleus. There, it forms an active HIF-1 complex by binding with HIF-1β, which then binds to hypoxia response elements (HRE) in the promoters of target genes, activating the transcription of over 40 genes involved in processes such as oxygen transport, glucose metabolism, cell survival, and angiogenesis. HIF-1α is highly expressed in many tissues, especially in the kidneys and heart, and is often overexpressed in various cancers, contributing to tumor growth and resistance to therapies. Its stability and activity are regulated by various post-translational modifications, including hydroxylation, acetylation, and phosphorylation.

免疫印迹
- WB result of HIF-1α Recombinant Rabbit mAb
  Primary antibody: HIF-1α Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Raji whole cell lysate 20 µg
  Lane 2: Raji treated with 100 μM CoCl₂ for4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 120 kDa
免疫细胞化学
- ICC analysis of HeLa cells treated with cobalt chloride (500μM, 6h) (top panel) and untreated HeLa cells (below panel). Anti- HIF-1α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on Raji+CoCl2(100μM, 4h)
  cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used HIF-1α Recombinant Rabbit mAb (S-3123) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of ARRDC3 and SAT-α in HIF-1 alpha Recombinant
  Rabbit mAb (S-3123)-immunoprecipitated sample.