热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

所有图片（1/17）

Histone H3 (mono methyl K36) Recombinant Rabbit mAb (S-859-505)

H3K36me

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6229
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (mono methyl K36)

  • 分子别名

    H3K36me

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-859-505

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    甲基化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, MCF7, Jurkat, NIH/3T3, Neuro-2a, mouse liver, PC-12, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt
    IHC-P 1:5000 Hu, Ms, Rt
    ICC 1:500 Hu
    ICFCM 1:500 Hu
    ChIP 1:20-1:50 Hu
背景介绍

  • Histone H3 (mono methyl K36) refers to a variant of histone H3 where the 36th lysine residue (K36) undergoes monomethylation, a crucial epigenetic regulatory mark. This modification is catalyzed by histone methyltransferases (e.g., SETD2, NSD1) and dynamically regulated by demethylases (e.g., KDM2A). H3K36me1 primarily localizes to gene promoters and enhancer regions, where it associates with transcriptional activation. It plays key roles in maintaining chromatin accessibility, facilitating transcriptional elongation, and promoting DNA damage repair. Studies have linked aberrant H3K36me1 distribution to cancers (e.g., leukemia, glioblastoma) and developmental disorders.

  • 免疫印迹

    • WB result of Histone H3 (mono methyl K36) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 18 kDa

    • WB result of Histone H3 (mono methyl K36) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 18 kDa

    • WB result of Histone H3 (mono methyl K36) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 18 kDa

  • 流式分析

    • Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labelling Histone H3 (mono methyl K36) antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-Histone H3 (mono methyl K36) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (mono methyl K36) Recombinant Rabbit mAb (S-859-505) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in Histone H3 (mono methyl K36)
      Recombinant Rabbit mAb (S-859-505)-immunoprecipitated
      sample.

  • 组织表达图谱

    • Expression of Histone H3 (mono methyl K36) in tumor tissue.

    • Expression of Histone H3 (mono methyl K36) in human tissue.

    • Expression of Histone H3 (mono methyl K36) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)