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Rabbit抗原名称
KCNB1分子别名
Potassium voltage-gated channel subfamily B member 1; Delayed rectifier potassium channel 1 (DRK1; h-DRK1); Voltage-gated potassium channel subunit Kv2.1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q14721克隆号
S-2527-21抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu, Ms, Rt ICC 1:50 Hu
KCNB1, also known as Kv2.1, is a 96-kDa, 858-amino-acid voltage-gated delayed-rectifier potassium channel α-subunit encoded on chromosome 20q13.3 that forms homo- or heterotetrameric pores (with silent α-subunits like KCNG, KCNV, KCNS families and regulatory β-subunits such as KCNE1-3) responsible for mediating outward K+ currents essential for repolarization of excitable membranes during high-frequency firing . The channel is enriched in somatodendritic domains of cortical and hippocampal neurons, pancreatic β-cells, heart, lungs and retina, and its gating, trafficking and clustering are dynamically regulated by phosphorylation/dephosphorylation of its cytoplasmic N- and C-termini in response to neuronal activity, transmitters, Ca2+/calcineurin signalling and oxidative stress, thereby controlling neuronal excitability, insulin secretion and apoptosis . Pathogenic KCNB1 variants produce epileptic encephalopathy, developmental delay and motor impairment by disrupting ion selectivity or inducing gain-of-function cation conductance.
免疫印迹
WB result of KCNB1 Recombinant Rabbit mAb
Primary antibody: KCNB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 96 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KCNB1 antibody was used at 1/××× dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCNB1 antibody was used at 1/××× dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCNB1 antibody was used at 1/××× dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SH-SY5Y cells. Anti-KCNB1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of KCNB1 in tumor tissue.
Expression of KCNB1 in human tissue.
Expression of KCNB1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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