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Rabbit抗原名称
LMPTP分子别名
Low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase; Red cell acid phosphatase 1; ACP1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P24666抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK-293, K562, HeLa, HepG2, NIH/3T3, mouse brain, C6, rat liver, COS-7预测反应种属
(反应种属缩写表)Ck, Pg, Or, Bv, Dr纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu ICC 1:500 Hu
Low molecular weight protein tyrosine phosphatase (LMPTP), an 18 kDa cytosolic class II PTP encoded by the ACP1 gene, exists as two splice isoforms (LMPTP-A and LMPTP-B), is ubiquitously expressed with especially high levels in adipocytes, and functions as a negative regulator of insulin signalling by dephosphorylating the insulin receptor and its substrates; human genetic studies show that low-activity ACP1 alleles are associated with reduced glycaemia, while experimental knockdown or genetic deletion of LMPTP in obese mice improves glucose tolerance and insulin sensitivity—mainly via hepatic effects—demonstrating that LMPTP drives obesity-linked insulin resistance and type 2 diabetes, thus representing an attractive drug target for metabolic disorders.
免疫印迹
WB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDaWB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17, 18 kDaWB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17, 18 kDaWB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HEK293 cells. Anti-LMPTP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of LMPTP in tumor tissue.
Expression of LMPTP in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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