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Rabbit抗原名称
Pex14分子别名
Peroxisomal membrane protein PEX14; PTS1 receptor-docking protein; Peroxin-14; Peroxisomal membrane anchor protein PEX14; PEX14免疫原
Synthetic PeptideAccession
O75381克隆号
S-2379-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, A431, HeLa, 293T, HepG2纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100 Hu ICC 1:50 Hu
PEX14, encoded by the PEX14 gene, is an integral peroxisomal membrane protein whose C-terminus faces the cytosol and constitutes the docking site for the PTS1 receptor PEX5 (and, indirectly, the PTS2 receptor PEX7), thereby nucleating the PEX13-PEX14 translocon pore that imports peroxisomal matrix proteins; beyond its essential role in peroxisome biogenesis, PEX14 also binds microtubules to drive organelle motility, functions as a transcriptional corepressor by recruiting histone deacetylases, and—uniquely among peroxins—is strictly required for selective autophagic degradation of peroxisomes (pexophagy), while mutations in PEX14 produce a Zellweger-syndrome phenotype .
免疫印迹
WB result of Pex14 Recombinant Rabbit mAb
Primary antibody: Pex14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 62 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Pex14 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Pex14 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Pex14 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-Pex14 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Pex14 in tumor tissue.
Expression of Pex14 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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