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Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb (S-3304)

Glycogen synthase kinase-3 alpha,GSK-3 alpha,Serine/threonine-protein kinase GSK3A,GSK3A,Glycogen synthase kinase-3 beta,GSK-3 beta,Serine/threonine-protein kinase GSK3B,GSK3B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6222

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-GSK3a/β (Ser21/9)
分子别名

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B
细胞定位

Nucleus, Mitochondrion, Synapse, Cytoplasm
Accession

P49840, P49841
克隆号

S-3304
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms

背景介绍

Phospho-GSK3α/β (Ser21/9) is the phosphorylated, inactive form of glycogen synthase kinase-3 alpha and beta isoforms, generated when AKT, MAPK and other kinases add a phosphate to Ser21 of GSK3α or the homologous Ser9 of GSK3β, thereby creating an N-terminal pseudo-substrate that folds back into the catalytic pocket to block substrate access and halt the enzyme’s constitutive suppression of glycogen synthesis, β-catenin signaling, NF-κB activity and other metabolic/transcriptional programs; detection of this phospho-epitope therefore serves as a widely used biomarker for PI3K–AKT–mTOR pathway activation and for cellular responses to insulin, growth factors, oxidative stress and Wnt stimuli.

免疫印迹
- WB result of Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated 293T whole cell lysate 20 µg
  Lane 2: 293T treated with 100 nM insulin for 20 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 45, 53 kDa
- WB result of Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Primary antibody: Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve overnight, then treated with 100 ng/ml PDGF for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 45, 53 kDa
  This blot was developed with high sensitivity substrate