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宿主来源
Rabbit抗原名称
Lamin A/C分子别名
Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32Accession
P02545克隆号
SDT-R041抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein A浓度
1 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:1000 Hu
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome.
流式分析
Flow cytometric analysis of Lamin A/C expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) or Jurkat (Human T cell leukemia T lymphocyte, Left) was stained with Phycoerythrin Rabbit IgG Isotype Control (Black line histogram) or SDT Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) Lamin A/C Recombinant Rabbit mAb (PE Conjugate) (Red line histogram) at 1/1000 dilution (0.1 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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