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Rabbit抗原名称
Akt (E17K Mutant Specific)分子别名
RAC-alpha serine/threonine-protein kinase; Protein kinase B (PKB); Protein kinase B alpha (PKB alpha); Proto-oncogene c-Akt; RAC-PK-alpha; PKB; RAC; AKT1免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, NucleusAccession
P31749抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
The AKT1(E17K mutant protein) is a constitutively active serine/threonine kinase generated by a single glutamate-to-lysine substitution at position 17 within its pleckstrin homology (PH) domain; this mutation creates new hydrogen bonds that markedly increase affinity for membrane phosphoinositides, driving persistent plasma-membrane localization and PI3K-independent phosphorylation of T308 and S473, thereby locking the kinase in an “always-on” conformation that fuels oncogenic signaling through mTORC1, GSK3β and FOXO, promotes cell survival, proliferation and migration, and confers both tumorigenic potential and resistance to many allosteric AKT inhibitors while remaining selectively targetable by emerging ATP-competitive or covalent mutant-specific inhibitors.
免疫印迹
WB result of Akt (E17K Mutant Specific) Rabbit pAb
Primary antibody: Akt (E17K Mutant Specific) Rabbit pAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with wild-type Akt1 whole cell lysate 20 µg
Lane 3: 293T transfected with E17K mutant Akt1 whole cell lysate 20 µg
Lane 4: 293T transfected with wild-type Akt2 whole cell lysate 20 µg
Lane 5: 293T transfected with E17K mutant Akt2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 62 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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