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Rabbit抗原名称
ELP3分子别名
Elongator complex protein 3; hELP3; tRNA uridine(34) acetyltransferase免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
Q9H9T3抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, MCF7, K562, Jurkat, Neuro-2a, C6, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:100 Hu, Ms
ELP3 (Elongator complex protein 3, also known as KAT9) is the catalytic histone acetyltransferase subunit of the highly conserved Elongator complex (composed of ELP1–ELP6) that associates with RNA polymerase II to facilitate transcriptional elongation via acetylation of histones H3 and H4, while also exhibiting cytoplasmic roles in α-tubulin acetylation to regulate neuronal migration and axonal branching, modifying wobble uridines in tRNAs to enhance translational efficiency, and participating in DNA demethylation after fertilization, cell migration, and redox homeostasis through acetylation of glucose-6-phosphate dehydrogenase, with its dysfunction implicated in neurodevelopmental disorders and tumor progression.
免疫印迹
WB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDaWB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrateWB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDaWB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- ELP3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Neuro-2a cells. Anti- ELP3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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