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Rabbit抗原名称
YTHDF1分子别名
YTH domain-containing family protein 1; Dermatomyositis associated with cancer putative autoantigen 1 homolog (DACA-1 homolog); Ythdf1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P59326克隆号
S-2443-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NIH/3T3, PC-12, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250-1:1000 Hu, Ms,Rt IF 1:500 Ms
YTHDF1 (YTH N6-methyladenosine RNA binding protein F1) is a cytoplasmic reader of the m6A epitranscriptomic mark that selectively binds N6-methylated adenosines within mRNAs and, by recruiting eIF3 and other translation initiation factors, enhances the ribosome loading and protein synthesis of its target transcripts, thereby influencing cell proliferation, synaptic plasticity, immune responses and tumorigenesis; structurally it contains an N-terminal intrinsically disordered region and a C-terminal YTH domain that confers high-affinity m6A recognition, and its expression or phosphorylation state is frequently dysregulated in multiple cancers, making it an emerging therapeutic target for small-molecule or oligonucleotide-based interventions.
免疫印迹
WB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDaWB result of YTHDF1 Recombinant Rabbit mAb
Primary antibody: YTHDF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-YTHDF1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-YTHDF1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-YTHDF1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- YTHDF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of YTHDF1 in human tissue.
Expression of YTHDF1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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