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Rabbit抗原名称
UBR2分子别名
E3 ubiquitin-protein ligase UBR2; N-recognin-2; Ubiquitin-protein ligase E3-alpha-2; Ubiquitin-protein ligase E3-alpha-II; C6orf133; KIAA0349; UBR2免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q8IWV8克隆号
S-2041-163抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, Jurkat, BxPC-3, HeLa, NIH/3T3, mouse liver, C6, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:100 Hu ICFCM 1:2000 Hu
UBR2 is an evolutionarily conserved E3 ubiquitin ligase of the N-end rule pathway that selectively recognizes and ubiquitinates proteins bearing specific N-terminal destabilizing residues, thereby targeting them for proteasomal degradation; it contains an N-terminal UBR box that binds type-1 (basic) and type-2 (bulky hydrophobic) N-degrons, a central RING finger domain that coordinates ubiquitin-charged E2 enzymes, multiple auto-inhibitory and substrate-recognition motifs, and C-terminal regions that mediate interactions with transcription factors, chromatin remodelers, and DNA damage response proteins, enabling UBR2 to regulate diverse processes such as spermatogenesis, neurogenesis, cardiac development, genomic stability, and metabolic homeostasis through both proteolytic and non-proteolytic ubiquitylation events.
免疫印迹
WB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDaWB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDaWB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDaWB result of UBR2 Recombinant Rabbit mAb
Primary antibody: UBR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) labelling UBR2 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in 293T cells. Anti-UBR2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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