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宿主来源
Rabbit抗原名称
Fbx32分子别名
F-box only protein 32; Atrogin-1; Muscle atrophy F-box protein (MAFbx); FBXO32免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q969P5克隆号
S-2442-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
mouse skeletal muscle, mouse heart预测反应种属
(反应种属缩写表)Pg,Rt,Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
Fbx32, also known as Atrogin-1 or MAFbx, is an F-box protein that functions as the substrate-recognition component of the Skp1-Cul1-F-box (SCF) E3 ubiquitin ligase complex, selectively targeting key muscle proteins such as MyoD, eIF3-f, and calcineurin for polyubiquitination and proteasomal degradation, thereby regulating skeletal muscle atrophy in response to catabolic signals like glucocorticoids, inflammatory cytokines, and denervation, while its expression is controlled by the FoxO transcription factors and its inhibition or deletion attenuates muscle wasting in various disease models.
免疫印迹
WB result of Fbx32 Recombinant Rabbit mAb
Primary antibody: Fbx32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 38 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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