分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
LIN28B分子别名
Protein lin-28 homolog B; Lin-28B; CSDD2免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
Q6ZN17克隆号
S-2360-117抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, K562, HEK-293, JAR纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
LIN28B is an evolutionarily-conserved RNA-binding protein that acts as a potent developmental timing regulator and oncofetal factor by selectively binding and blocking the biogenesis of let-7 family microRNAs, thereby relieving repression of pluripotency, cell-cycle, and metabolic genes; it is predominantly expressed during embryogenesis and in many cancers, where its overexpression promotes self-renewal, metabolic reprogramming, and aggressive phenotypes through both let-7–dependent and independent mechanisms, including direct stabilization of IGF2, HMGA1, and MYC mRNAs and activation of the PI3K/AKT/mTOR and WNT/β-catenin pathways, making LIN28B a critical driver of oncogenesis, stemness, and resistance to therapy across diverse tumor types.
免疫印迹
WB result of LIN28B Recombinant Rabbit mAb
Primary antibody: LIN28B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: JAR whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 33 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-LIN28B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-LIN28B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-LIN28B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in K562 cells. Anti-LIN28B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HepG2 cells. Anti-LIN28B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of LIN28B in tumor tissue.
Expression of LIN28B in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)