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MRP2 Recombinant Rabbit mAb (S-2316-41)

ATP-binding cassette sub-family C member 2,Canalicular multidrug resistance protein,Canalicular multispecific organic anion transporter 1,Multidrug resistance-associated protein 2,CMOAT,CMOAT1,CMRP,ABCC2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6203

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

MRP2
分子别名

ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; Multidrug resistance-associated protein 2; CMOAT; CMOAT1; CMRP; ABCC2
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

Q92887
克隆号

S-2316-41
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

A549, HepG2, A204, mouse liver
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:1000 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:1000	Hu

背景介绍

MRP2 (Multidrug Resistance-associated Protein 2), also known as ABCC2, is a 1,545-amino-acid ATP-binding cassette (ABC) transporter localized almost exclusively to the apical (canalicular) membrane of hepatocytes, where it functions as the primary efflux pump for conjugated organic anions including bilirubin glucuronides, glutathione conjugates, and a wide array of xenobiotics such as anticancer drugs, antibiotics, and environmental toxins, thereby playing a central role in hepatic detoxification and limiting the systemic exposure of tissues to these compounds; genetic mutations in the ABCC2 gene lead to Dubin–Johnson syndrome, a benign autosomal recessive disorder characterized by conjugated hyperbilirubinemia and black liver, whereas acquired MRP2 down-regulation contributes to cholestasis and multidrug resistance in cancer chemotherapy, and its expression is transcriptionally regulated by nuclear receptors like PXR, CAR, and FXR that respond to endobiotics and xenobiotics, making MRP2 a critical determinant of pharmacokinetics, drug–drug interactions, and bile formation.

免疫印迹
- WB result of MRP2 Recombinant Rabbit mAb
  Primary antibody: MRP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled HeLa whole cell lysate 20 µg
  Lane 2: unboiled A549 whole cell lysate 20 µg
  Lane 3: unboiled HepG2 whole cell lysate 20 µg
  Lane 4: unboiled A204 whole cell lysate 20 µg
  Negative control: unboiled HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 174 kDa
  Observed MW: 250-400 kDa
- WB result of MRP2 Recombinant Rabbit mAb
  Primary antibody: MRP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 174 kDa
  Observed MW: 250-400 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-MRP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of MRP2 in tumor tissue.
- Expression of MRP2 in human tissue.