PPIA Recombinant Rabbit mAb (S-2406-28)

Peptidyl-prolyl cis-trans isomerase A,PPIase A,Cyclophilin A,Cyclosporin A-binding protein,Rotamase A,CYPA

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6202

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

PPIA
分子别名

Peptidyl-prolyl cis-trans isomerase A; PPIase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; CYPA
免疫原

Synthetic Peptide
细胞定位

Nucleus, Secreted, Cytoplasm
Accession

P62937
克隆号

S-2406-28
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

K562, HeLa, Jurkat, HepG2, A431, NIH/3T3, EL4, C2C12, mouse brain, mouse heart, mouse kidney, mouse spleen
预测反应种属
(反应种属缩写表)

Ct, Bv, CtTa
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms
IHC-P 1:250 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ICFCM 1:500 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms
IHC-P	1:250	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ICFCM	1:500	Hu, Ms

背景介绍

PPIA (peptidyl-prolyl cis-trans isomerase A), also called cyclophilin A, is a 165-amino-acid, ubiquitously expressed cytoplasmic enzyme that catalyzes the cis-trans isomerization of prolyl peptide bonds and thus accelerates protein folding; beyond this chaperone-like role, it is a critical virulence factor for HIV-1, because the viral capsid recruits PPIA to shield itself from host restriction factors, while extracellular PPIA, secreted under inflammatory or oxidative stress, acts as a chemoattractant for monocytes and T cells via CD147/EMMPRIN, thereby amplifying inflammatory cascades in cardiovascular disease, viral infection, and cancer.

免疫印迹
- WB result of PPIA Recombinant Rabbit mAb
  Primary antibody: PPIA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 18 kDa
- WB result of PPIA Recombinant Rabbit mAb
  Primary antibody: PPIA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: EL4 whole cell lysate 20 µg
  Lane 3: C2C12 whole cell lysate 20 µg
  Lane 4: mouse brain lysate 20 µg
  Lane 5: mouse heart lysate 20 µg
  Lane 6: mouse kidney lysate 20 µg
  Lane 7: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 20 kDa
流式分析
- Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast) labelling PPIA antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) labelling PPIA antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-PPIA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in K562 cells. Anti- PPIA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in K562 cells. Anti- PPIA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
组织表达图谱
- Expression of PPIA in tumor tissue.
- Expression of PPIA in human tissue.
- Expression of PPIA in mouse & rat tissue.