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Rabbit抗原名称
PRKCQ分子别名
Protein kinase C theta type; nPKC-theta; PRKCT免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
Q04759克隆号
S-2511-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
Jurkat, K562, Ramos, mouse spleen, mouse thymus纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
Protein kinase C theta (PKC-θ), encoded by the PRKCQ gene on chromosome 10p15.1, is a calcium-independent, phospholipid-dependent serine/threonine kinase selectively expressed in hematopoietic cells—especially platelets and T lymphocytes—where it transduces T-cell receptor signals to activate NF-κB and AP-1, thereby orchestrating T-cell activation, survival, and distinct Th17-mediated immune responses, while also promoting oncogenic growth and anoikis resistance in triple-negative breast cancer cells, and its kinase activity is considered a potential therapeutic target in autoimmunity and cancer.
免疫印迹
WB result of PRKCQ Recombinant Rabbit mAb
Primary antibody: PRKCQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Low expression control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDaWB result of PRKCQ Recombinant Rabbit mAb
Primary antibody: PRKCQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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