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Rabbit抗原名称
Snail分子别名
Zinc finger protein SNAI1; Protein snail homolog 1 (Protein sna); SNAH; SNAI1细胞定位
Cytoplasm, NucleusAccession
O95863克隆号
S-3257抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
HCT 116, HeLa , A549, SW480, NIH/3T3, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Mk
Snail protein, a zinc-finger transcription factor encoded by the SNAI1 gene, is a master regulator of epithelial-to-mesenchymal transition (EMT) that represses E-cadherin and other epithelial genes, thereby promoting cell motility, invasion, and stemness, and its overexpression in diverse carcinomas is strongly linked to metastasis, chemoresistance, and poor prognosis through activation of oncogenic signaling pathways such as TGF-β, Wnt/β-catenin, and NF-κB, while its activity is modulated by post-translational modifications, including phosphorylation, ubiquitination, and acetylation, making it both a biomarker and a therapeutic target in cancer biology.
免疫印迹
WB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrateWB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
This blot was developed with high sensitivity substrateWB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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