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CXCL1 Recombinant Rabbit mAb (S-1856-35)

Growth-regulated alpha protein,C-X-C motif chemokine 1,Platelet-derived growth factor-inducible protein KC,Secretory protein N51,Gro,Gro1,Mgsa,Scyb1,Cxcl1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6193

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CXCL1
分子别名

Growth-regulated alpha protein; C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; Gro; Gro1; Mgsa; Scyb1; Cxcl1
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P12850
克隆号

S-1856-35
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Ms
ICC 1:500 Ms
ICFCM 1:500 Ms

应用	稀释度	推荐种属
WB	1:1000-1:5000	Ms
ICC	1:500	Ms
ICFCM	1:500	Ms

背景介绍

CXCL1, also known as Keratinocyte Chemoattractant (KC), is a chemokine protein that plays a crucial role in immune responses. It is primarily produced by various cell types, including macrophages and endothelial cells, in response to inflammatory stimuli. CXCL1/KC functions by attracting neutrophils to sites of infection or tissue damage, thereby facilitating the immune system's ability to combat pathogens and initiate the healing process. This protein is also involved in the regulation of cell proliferation and angiogenesis, making it an important factor in both acute and chronic inflammatory conditions. Its expression and activity are tightly regulated to ensure appropriate immune responses and tissue homeostasis.

免疫印迹
- WB result of CXCL1 Recombinant Rabbit mAb
  Primary antibody: CXCL1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated RAW264.7 whole cell lysate 20 µg
  Lane 2: RAW264.7 treated with 100 ng/ml LPS for 6 hours, then add 300 ng/ml Brefeldin A for 3 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 10 kDa
  Observed MW: 11 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed and Fixation/Permeabilization solution permeabilized RAW264.7, Stimulated 6 hours with 100ng/ml LPS and the last 3 hours with 300ng/ml BFA (Red) or untreated (Left panel) labelling CXCL1 antibody at 1/500 dilution (0.1 μg). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC analysis of Raw264.7 cells treated with LPS (100 ng/ml, 6h) and Brefeldin A (300 ng/ml, 3 h) (top panel) and untreated Raw264.7 cells (below panel). Anti-CXCL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).