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Rabbit抗原名称
Fibrillarin分子别名
rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase; U6 snRNA 2'-O-methyltransferase fibrillarin; FIB1; FLRN; FBL免疫原
Synthetic Peptide细胞定位
NucleusAccession
P22087克隆号
S-1718-171抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK-293, HeLa, HepG2, Molt-4, Jurkat, NIH/3T3, mouse liver, C6, rat testis, COS-7预测反应种属
(反应种属缩写表)Dr, Xe, Fu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:50-1:100 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:50 Hu
Fibrillarin is an evolutionarily conserved, 34-kDa nucleolar methyltransferase that is essential for 2'-O-methylation of pre-ribosomal RNA and pre-assembly of small nucleolar ribonucleoprotein (snoRNP) complexes on nascent ribosomal RNA, thereby driving early steps of ribosome biogenesis; it contains an N-terminal glycine- and arginine-rich (GAR) domain that mediates nucleolar localization and interaction with box C/D snoRNAs, followed by a central RNA-binding domain and a C-terminal S-adenosyl-L-methionine-dependent methyltransferase motif, localizes predominantly to the dense fibrillar component of the nucleolus in an Nop56/Nop58- and 15.5 kDa protein-dependent manner, and is recruited to sites of rDNA transcription by UBF and SL1; mutations or depletion of fibrillarin impair rRNA processing, leading to defects in 18S and 28S rRNA maturation, nucleolar stress, cell-cycle arrest, and embryonic lethality in model organisms, while in humans its overexpression is linked to several cancers and its absence causes the neurodevelopmental disorder Néstor-Guillermo progeria syndrome, underscoring its dual role as a guardian of ribosome production and a sensor of nucleolar integrity.
免疫印迹
WB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 35 kDaWB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDaWB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDaWB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Fibrillarin antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Fibrillarin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Fibrillarin in tumor tissue.
Expression of Fibrillarin in human tissue.
Expression of Fibrillarin in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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