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Rabbit抗原名称
GSDMD分子别名
Gasdermin-D; Gasdermin domain-containing protein 1; DFNA5L; GSDMDC1细胞定位
Cytoplasm, Secreted, Mitochondrion, Cell membraneAccession
P57764克隆号
S-3267抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu
Gasdermin D (GSDMD) is a crucial protein within the gasdermin family, playing a key role in executing pyroptosis, a form of lytic, inflammatory cell death. The full-length GSDMD protein is inactive and consists of a 31 kD N-terminal pore-forming domain and a 22 kD C-terminal suppression domain. Upon activation by caspases such as caspase-1, -4, -5, or -11, the interdomain linker is cleaved, releasing the N-terminal fragment (GSDMD-NT) from the inhibitory C-terminal domain. The freed GSDMD-NT translocates to the plasma membrane, oligomerizes, and forms pores with an inner diameter of approximately 10–14 nm, leading to cell lysis, cytokine release (such as IL-1β and IL-18), and disruption of ion and water balance. This pore formation is a critical step in mediating inflammation and immune responses. GSDMD is also implicated in various diseases, including sepsis, myocardial ischemia/reperfusion injury, and certain cancers. Research on selective GSDMD inhibitors is ongoing, aiming to block pyroptosis and reduce inflammation in pathological conditions.
免疫印迹
WB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDaWB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDaWB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GSDMD in tumor tissue.
Expression of GSDMD in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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