PI3 Kinase p110α Recombinant Rabbit mAb (S-3130)
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform,PI3-kinase subunit alpha,PI3K-alpha,PI3Kalpha,PtdIns-3-kinase subunit alpha,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha,p110alpha),Phosphoinositide 3-kinase alpha,Phosphoinositide-3-kinase catalytic alpha polypeptide,Serine/threonine protein kinase PIK3CA,PIK3CA
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宿主来源
Rabbit抗原名称
PI3 Kinase p110α分子别名
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha; p110alpha); Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA; PIK3CA细胞定位
CytoplasmAccession
P42336克隆号
S-3130抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NIH/3T3, C2C12, mouse brain预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
PI3 Kinase p110α (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha) is a 1,068-amino-acid lipid kinase that, in complex with its regulatory subunit p85, phosphorylates PIP₂ to generate the second messenger PIP₃, thereby activating the PI3K/AKT/mTOR pathway that controls cell growth, survival, metabolism, and angiogenesis; encoded by PIK3CA, it contains an N-terminal adaptor-binding domain, a Ras-binding domain, a C2 domain, a helical domain, and a C-terminal kinase domain, and is the most frequently mutated oncogene in human cancers, with hotspot mutations (e.g., E545K and H1047R) causing constitutive lipid kinase activity and driving tumorigenesis.
免疫印迹
WB result of PI3 Kinase p110α Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 110 kDaWB result of PI3 Kinase p110α Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 110 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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