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TdT Recombinant Rabbit mAb (S-2364-59)

DNA nucleotidylexotransferase,Terminal addition enzyme,Terminal deoxynucleotidyltransferase (Terminal transferase),TDT,DNTT

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6182

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

TdT
分子别名

DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

P04053
克隆号

S-2364-59
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

Jurkat, Molt-4
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
ICC 1:500 Hu
ICFCM 1:50 Hu

背景介绍

Terminal deoxynucleotidyl transferase (TdT), also called DNA nucleotidylexotransferase or terminal transferase, is a template-independent DNA polymerase encoded by the DNTT gene on human chromosome 10q23-q24 that is selectively expressed in immature lymphoid precursors (pre-B and pre-T cells) and certain leukemias/lymphomas; it catalyzes the non-templated addition of deoxynucleotides to the 3'-OH ends of DNA, generating N-region diversity at V(D)J junctions during immunoglobulin and T-cell receptor gene rearrangement, thereby expanding antigen-receptor repertoires critical for adaptive immunity, and can utilize a broad range of divalent cations (Mg²⁺, Mn²⁺, Zn²⁺, Co²⁺) as cofactors .

免疫印迹
- WB result of TdT Recombinant Rabbit mAb
  Primary antibody: TdT Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: Molt-4 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 56 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) labelling TdT antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in MOLT-4 cells. Anti- TdT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).