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宿主来源
Rabbit抗原名称
teraspanin-33分子别名
Tspan-33; Penumbra (hPen); Proerythroblast new membrane; PEN; TSPAN33免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
Q86UF1克隆号
S-1953-44抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, Ramos, Daudi预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu
Tetraspanin-33 (Tspan-33, encoded by TSPAN33, also called Penumbra) is a four-transmembrane-domain protein of the tetraspanin superfamily that is virtually absent from resting B cells but strongly induced upon activation, localizes to membrane microvilli, the Golgi and extracellular vesicles, and functions as a key regulator of human B-lymphocyte endocytosis, adhesion and migration by modulating plasma-membrane tension and cytoskeletal dynamics; it is furthermore expressed in several lymphomas and autoimmune diseases, qualifying it as a potential diagnostic biomarker and therapeutic target for malignant B cells.
免疫印迹
WB result of Teraspanin-33 Recombinant Rabbit mAb
Primary antibody: Teraspanin-33 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Raji whole cell lysate 20 µg
Lane 2: unboiled Ramos whole cell lysate 20 µg
Lane 3: unboiled Daudi whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 35 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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