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Rabbit抗原名称
TGF beta 1分子别名
Transforming growth factor beta-1 proprotein; TGFB; TGFB1细胞定位
SecretedAccession
P01137克隆号
S-3204抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, A549, HL-60, SH-SY5Y, NIH/3T3, RAW264.7, mouse spleen, C6, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Transforming Growth Factor-beta 1 (TGF-β1) is a multifunctional 25 kDa homodimeric cytokine that belongs to the TGF-β superfamily and is secreted by virtually all somatic cells as a large latent complex bound to latency-associated peptide and latent TGF-β binding protein; upon activation by proteases or integrins it signals through a heteromeric receptor complex of TβRII and ALK5 (TβRI) serine/threonine kinases to phosphorylate Smad2/3, which then complex with Smad4 to regulate transcription of hundreds of genes that orchestrate pleiotropic effects ranging from embryogenesis, extracellular matrix deposition, immune suppression and wound healing to cell cycle arrest, apoptosis, and epithelial-mesenchymal transition, with dysregulation implicated in fibrosis, cancer, and autoimmune disease.
免疫印迹
WB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrateWB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrateWB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human bone marrow. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TGF beta 1 in tumor tissue.
Expression of TGF beta 1 in human tissue.
Expression of TGF beta 1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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