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Rabbit抗原名称
TMEM106B分子别名
Transmembrane protein 106B免疫原
Recombinant Protein细胞定位
Cell membrane, Lysosome, EndosomeAccession
Q9NUM4克隆号
S-2362-33抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
PANC-1, HeLa, A549, HepG2, Jurkat, NIH/3T3, mouse brain, PC-12, rat brain, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IP 1:500 Hu IHC-P 1:100-1:500 Hu, Ms, Rt
TMEM106B is a 274-amino-acid type II transmembrane protein embedded in late endosome and lysosome membranes, most highly expressed in central nervous system neurons and oligodendrocytes, where it critically influences lysosomal morphology, trafficking, and clearance of misfolded proteins; genetic variants—especially a threonine-to-serine polymorphism at residue 185—modulate its expression and stability and constitute major risk factors for frontotemporal lobar degeneration with TDP-43 pathology and other neurodegenerative disorders, while its C-terminal luminal domain can be proteolytically released by lysosomal cysteine proteases to form amyloid fibrils that accumulate not only in diseased but also in aged cognitively normal brains, thereby linking lysosomal dysfunction, myelin lipid dysregulation, and protein aggregation to neurodegeneration.
免疫印迹
WB result of TMEM106B Recombinant Rabbit mAb
Primary antibody: TMEM106B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Raji whole cell lysate 20 µg
Lane 2: unboiled PANC-1 whole cell lysate 20 µg
Negative control: unboiled Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40, 80 kDaWB result of TMEM106B Recombinant Rabbit mAb
Primary antibody: TMEM106B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HeLa whole cell lysate 20 µg
Lane 2: unboiled A549 whole cell lysate 20 µg
Lane 3: unboiled HepG2 whole cell lysate 20 µg
Lane 4: unboiled Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40, 80 kDaWB result of TMEM106B Recombinant Rabbit mAb
Primary antibody: TMEM106B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled NIH/3T3 whole cell lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40, 80 kDaWB result of TMEM106B Recombinant Rabbit mAb
Primary antibody: TMEM106B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled PC-12 whole cell lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40, 80 kDaWB result of TMEM106B Recombinant Rabbit mAb
Primary antibody: TMEM106B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40, 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-TMEM106B antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TMEM106B antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-TMEM106B antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-TMEM106B antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TMEM106B antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TMEM106B antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in PANC-1 cells (top panel) and negative staining in Raji cells (below panel). Anti-TMEM106B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TMEM106B in tumor tissue.
Expression of TMEM106B in human tissue.
Expression of TMEM106B in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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