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宿主来源
Rabbit抗体类型
Polyclonal antibody抗体同种型
IgG纯化方式
Immunogen Affinity浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ELISA
The anti-Cetuximab antibody is a monoclonal or polyclonal antibody that specifically recognizes and targets Cetuximab. It is primarily used in scientific research, clinical testing, and drug monitoring. Cetuximab is a chimeric monoclonal antibody drug targeting the Epidermal Growth Factor Receptor (EGFR), widely used in the treatment of various malignant tumors such as colorectal cancer and head and neck squamous cell carcinoma. By binding to specific epitopes of Cetuximab, the Anti-Cetuximab antibody can be employed in experimental techniques like Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, and Immunohistochemistry (IHC) to quantify Cetuximab concentration in biological samples, analyze its pharmacokinetic properties, or assess the potential development of anti-drug antibodies (ADA) in patients. Furthermore, this antibody aids in researching resistance mechanisms to Cetuximab, such as monitoring changes in the EGFR signaling pathway or the efficiency of drug-receptor binding, thereby providing a critical tool for optimizing targeted therapy regimens.
ELISA
Antigen (Cetuximab, human total IgG) 1μg/ml is coated overnight at 4°C. Anti- Cetuximab Rabbit polyclonal Antibody is added in serial dilutions and incubated at 37°C for 1 hours. Peroxidase Goat Anti-Rabbit IgG (min X Hu Sr Prot) secondary antibody is added at 37°C for 40 min. Substrate solution is added and developed for 5 minutes at room temperature. Absorbance is measured at 450 nm.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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