CACNA1S Recombinant Rabbit mAb (S-2448-78)
Voltage-dependent L-type calcium channel subunit alpha-1S,Calcium channel,L type,alpha-1 polypeptide,isoform 3,skeletal muscle,Voltage-gated calcium channel subunit alpha Cav1.1,CACH1,CACN1,CACNL1A3 s
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宿主来源
Rabbit抗原名称
CACNA1S分子别名
Voltage-dependent L-type calcium channel subunit alpha-1S; Calcium channel; L type; alpha-1 polypeptide; isoform 3; skeletal muscle; Voltage-gated calcium channel subunit alpha Cav1.1; CACH1; CACN1; CACNL1A3 s免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q13698克隆号
S-2448-78抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human skeletal muscle, mouse skeletal muscle, rat skeletal muscle预测反应种属
(反应种属缩写表)Rb纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:4000 Rt IHC-P 1:500 Hu, Ms, Rt
The CACNA1S protein is a crucial component of the voltage-dependent L-type calcium channel, specifically the alpha-1 subunit, which plays a vital role in excitable cells such as skeletal muscle and cardiac muscle. This protein is encoded by the CACNA1S gene and is responsible for regulating the flow of calcium ions into cells, thereby influencing muscle contraction and other physiological processes. Mutations in the CACNA1S gene can lead to various disorders, including hypokalemic periodic paralysis and malignant hyperthermia susceptibility, highlighting its significance in maintaining normal muscle function and homeostasis.
免疫印迹
WB result of CACNA1S Recombinant Rabbit mAb
Primary antibody: CACNA1S Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 212 kDa
Observed MW: 212 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-CACNA1S antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CACNA1S antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-CACNA1S antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-CACNA1S antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-CACNA1S antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CACNA1S in tumor tissue.
Expression of CACNA1S in human tissue.
Expression of CACNA1S in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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