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Erk1/2 Recombinant Rabbit mAb (S-3178)

Mitogen-activated protein kinase 1,MAP kinase 1,MAPK 1,ERT1,Extracellular signal-regulated kinase 2 (ERK-2),MAP kinase isoform p42 (p42-MAPK),Mitogen-activated protein kinase 2 (MAP kinase 2,MAPK 2),ERK2,PRKM1,PRKM2,MAPK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6166

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Erk1/2
分子别名

Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2; MAPK 2); ERK2; PRKM1; PRKM2; MAPK1
细胞定位

Cytoskeleton, Nucleus, Cytoplasm
Accession

P28482
克隆号

S-3178
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, K562, Daud, HepG2, U-87 MG, PC-3, NIH/3T3, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms, Rt, Mk
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

Erk1/2 proteins, also known as extracellular signal-regulated kinases 1 and 2, are key components of the MAPK (mitogen-activated protein kinase) signaling pathway. They play roles crucial in regulating various cellular processes such as cell proliferation, differentiation, survival, and apoptosis. Erk1/2 can be activated by a wide range of extracellular stimuli including growth factors, cytokines, and hormones. Once activated, they phosphorylate a cascade of downstream targets, thereby transmitting signals from the cell surface to the nucleus and modulating gene expression. Dysregulation of Erk1/2 signaling has been implicated in numerous diseases, including cancer andde neurogenerative disorders, making them important targets for therapeutic intervention.

免疫印迹
- WB result of Erk1/2 Recombinant Rabbit mAb
  Primary antibody: Erk1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: Daudi whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: U-87 MG whole cell lysate 20 µg
  Lane 6: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 40 kDa
- WB result of Erk1/2 Recombinant Rabbit mAb
  Primary antibody: Erk1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 39, 42 kDa
- WB result of Erk1/2 Recombinant Rabbit mAb
  Primary antibody: Erk1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 39, 42 kDa
- WB result of Erk1/2 Recombinant Rabbit mAb
  Primary antibody: Erk1/2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 40 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell) labelling Erk1/2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Erk1/2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Erk1/2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Erk1/2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Erk1/2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-Erk1/2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in U-87 MG cells. Anti-ERK1/2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Erk1/2 in tumor tissue.
- Expression of Erk1/2 in human tissue.
- Expression of Erk1/2 in mouse & rat tissue.