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宿主来源
Rabbit抗原名称
Hyaluronidase PH20/SPAM1分子别名
Hyaluronidase PH-20; Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20; HYAL3; PH20免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P38567克隆号
S-2143-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human testis, mouse testis, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
Hyaluronidase PH20, also known as SPAM1 (Sperm Adhesion Molecule 1), is a multifunctional glycosyl hydrolase enzyme belonging to the glycosyl hydrolase 56 family. It is a GPI-anchored protein expressed in the testis and found on the surface of mammalian sperm and the inner acrosomal membrane. PH20/SPAM1 is unique for its hyaluronidase activity at neutral pH, which is essential for sperm to penetrate the hyaluronic acid-rich cumulus cell layer surrounding the oocyte during fertilization. Additionally, it plays roles in zona pellucida binding and calcium signaling associated with acrosomal exocytosis. This enzyme is also involved in sperm maturation and has potential applications in reproductive medicine and contraception.
免疫印迹
WB result of Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb
Primary antibody: Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 57 kDaWB result of Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb
Primary antibody: Hyaluronidase PH20/SPAM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Hyaluronidase PH20/SPAM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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