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Rabbit抗原名称
Lipocalin-2/NGAL分子别名
Neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Oncogene 24p3; Siderocalin; p25; HNL; LCN2免疫原
Synthetic Peptide细胞定位
SecretedAccession
P80188克隆号
S-2279-72抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
COLO 205, SW480, A431纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000-1:2000 Hu
Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a multifunctional protein that belongs to the lipocalin family. It is primarily expressed in neutrophils and certain epithelial cells. NGAL plays a crucial role in various biological processes, including iron homeostasis, where it binds and sequesters iron-loaded bacterial siderophores to inhibit bacterial growth, thus contributing to the immune defense mechanism. Additionally, it is involved in cellular differentiation, proliferation, and apoptosis. In clinical contexts, NGAL has emerged as a biomarker for early detection of acute kidney injury, as its levels in urine and blood can rise rapidly in response to renal ischemia or nephrotoxic injury. Furthermore, it has been implicated in the progression of several cancers, where it may influence tumor growth, metastasis, and response to therapy, making it a potential target for cancer diagnostics and treatment.
免疫印迹
WB result of Lipocalin-2/NGAL Recombinant Rabbit mAb
Primary antibody: Lipocalin-2/NGAL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Negative control: 293T whole cell lysate; MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDaWB result of Lipocalin-2/NGAL Recombinant Rabbit mAb
Primary antibody: Lipocalin-2/NGAL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Lipocalin-2/NGAL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Lipocalin-2/NGAL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Lipocalin-2/NGAL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-Lipocalin-2/NGAL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Lipocalin-2/NGAL antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Lipocalin-2/NGAL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Lipocalin-2/NGAL in tumor tissue.
Expression of Lipocalin-2/NGAL in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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