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Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb (S-1810-101)

Glycogen synthase kinase-3 alpha,GSK-3 alpha,Serine/threonine-protein kinase GSK3A,GSK3A,Glycogen synthase kinase-3 beta,GSK-3 beta,Serine/threonine-protein kinase GSK3B,GSK3B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6159

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho - GSK-3α/β (Tyr216/279)
分子别名

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm, Nucleus
Accession

P49841, P49840
克隆号

S-1810-101
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:5000 Hu, Ms, Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000-1:5000	Hu, Ms, Rt

背景介绍

Phospho - GSK-3α/β (Tyr216/279) proteins are key players in cellular signaling pathways. Glycogen synthase kinase-3 (GSK-3) exists in two isoforms, α and β, and phosphorylation at specific tyrosine residues, such as Tyr216 in GSK-3β and Tyr279 in GSK-3α, can significantly impact their activity. This phosphorylation typically enhances the kinase activity of GSK-3, allowing it to regulate a wide range of biological processes including glycogen metabolism, gene transcription, and cell fate determination. Dysregulation of GSK-3 phosphorylation has been implicated in various diseases, making it an important target for therapeutic interventions.

免疫印迹
- WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
  Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: phosphatase treated HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 47, 51 kDa
- WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
  Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Lane 2: phosphatase treated mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 47, 51 kDa
- WB result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
  Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Lane 2: phosphatase treated rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 47, 51 kDa
斑点杂交
- Dot blot result of Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb
  Lane 1: GSK-3α/β (Tyr216/279) phospho peptide
  Lane 2: GSK-3α/β (Tyr216/279) unmodified peptide
  Primary antibody: Phospho - GSK-3α/β (Tyr216/279) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution