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Phospho-Lck (Tyr394) Recombinant Rabbit mAb (S-1957-74)

Tyrosine-protein kinase Lck,Leukocyte C-terminal Src kinase (LSK),Lymphocyte cell-specific protein-tyrosine kinase,Protein YT16,Proto-oncogene Lck,T cell-specific protein-tyrosine kinase,p56-LCK,LCK

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6158

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Lck (Tyr394)
分子别名

Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase (LSK); Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; LCK
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P06239
克隆号

S-1957-74
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Ck, CyMk, Dg, Pg, Xe, Zf
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:5000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000-1:5000	Hu, Ms
ICC	1:500	Hu

背景介绍

Phospho-Lck (Tyr394) protein is a phosphorylated form of the Lck protein at tyrosine residue 394. Lck, or lymphocyte-specific protein tyrosine kinase, is a non-receptor tyrosine kinase that plays a crucial role in the early stages of T-cell activation. When phosphorylated at Tyr394, Lck becomes fully activated, enabling it to initiate downstream signaling cascades. This phosphorylation event is essential for the proper functioning of the immune system, as it helps in the transmission of signals from the T-cell receptor to intracellular effectors, ultimately leading to T-cell proliferation and differentiation. Abnormal regulation of Phospho-Lck (Tyr394) has been implicated in various immune disorders, making it an important target for therapeutic interventions.

免疫印迹
- WB result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
  Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Jurkat whole cell lysate 5 µg
  Lane 2: Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate 5 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 55-70 kDa
- WB result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
  Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 55 kDa
斑点杂交
- Dot blot result of Phospho-Lck (Tyr394) Recombinant Rabbit mAb
  Lane 1: Lck (Tyr394) phospho peptide 1
  Lane 2: Lck (Tyr394) phospho peptide 2
  Lane 3: Lck (Tyr394) unmodified peptide
  Primary antibody: Phospho-Lck (Tyr394) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of Jurkat cells treated with 50mM pervanadate for 5 minutes (top panel) and untreated Jurkat cells (below panel). Anti- Phospho-Lck (Y394) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).